The mechanism of Polydatin in shock treatment

Abstract

Polydatin is extracted from a traditional Chinese herbal medicine, Polygonum cuspidatum, and has a special effect in shock treatment. The aim of this study is to explain the cellular and molecular basis of Polydatin in shock treatment. The fluorescent probe techniques, patch clamp method, and cellular flow chamber were used to test intracellular variables of vascular smooth muscle cells (VSMC), myocardial cells (MC), endothelial cells (EC), and white blood cell (WBC). It was shown that Polydatin could inhibit ICAM‐1 expression in EC stimulated by lipopolysaccharide (LPS), attenuate WBC‐EC adhesion, increase [Ca $^{2+}]\tsub{i}$ in MC with enhancement of MC contraction extent, activate K_ATP channels of VSMC, and decrease pH_i value and [Ca $^{2+}]\tsub{i}$ of VSMC in shock. The study suggests that Polydatin has multiple effects on VSMC, MC, WBC and EC, which are related to the enhancement of heart function and improvement of microcirculatory perfusion in shock.