Modulation of pulmonary oxidative status in methamphetamine-withdrawn rats,comparing the effects of continuous training and NBS superfood supplementation
Restricted accessResearch articleFirst published online December 3, 2024
Modulation of pulmonary oxidative status in methamphetamine-withdrawn rats,comparing the effects of continuous training and NBS superfood supplementation
This study aimed to investigate the effects of six weeks of continuous training and Nutrition Bio-shield (NBS) Superfood Supplementation on the state of oxidative stress by the expression of Nrf2, NOX4, superoxide dismutase, and malondialdehyde genes in the lungs of rats after methamphetamine withdrawal.
METHODS:
Forty male Wistar rats were randomly divided into five groups (n = 8, per group), undergoing methamphetamine administration (six weeks, 5 mg/kg ip, and once per day) followed by a 21-day withdrawal period. The rats were supplemented NBS superfood at a dosage of 25 g/kg per day for six weeks. The training protocol was 30 minutes of daily continuous training (treadmill running), five days a week for six weeks. The regimen escalated from a pace of 3 m/min for the initial 5 minutes, to 5 m/min for the following 5 minutes, culminating at 8 m/min for the remainder of the session, all at a 0° incline. A one-way analysis of variance was performed to analyze the gene expression of Nrf2, NOX4, MDA, and SOD in the lungs tissue of rats.
RESULTS:
The results indicated that, in the experimental groups which underwent continuous training and NBS Superfood supplementation, the expression of the Nrf2 gene exhibited a significant elevation compared to the control group (P < 0.05), while the NOX4, MDA, and SOD genes expression exhibited a significant decline in comparison to the control group (P < 0.05).
CONCLUSION:
In general, both exercise interventions and NBS superfood supplementation, when employed separately or in combination after methamphetamine withdrawal, can enhance the state of oxidative stress in the lung.
PatelD, DesaiGM, FrasesS, CorderoRJ, DeLeon-RodriguezCM, EugeninEA, et al.Methamphetamine enhances Cryptococcus neoformans pulmonary infection and dissemination to the brain. MBio. 2013;4(4):e00400–13. 10.1128/mbio.00400-13.
2.
Van HoutenB. Pulmonary Arterial hypertension is associated with oxidative stress– induced genome instability. American Thoracic Society; 2015. p. 129–30.
3.
Ushio-FukaiM. Localizing NADPH oxidase– derived ROS. Science’s STKE. 2006;2006(349):re8.
4.
AutenRL, DavisJM. Oxygen toxicity and reactive oxygen species: The devil is in the details. Pediatric Research. 2009;66(2):121–7.
5.
ZhangQ, GouF, ShiP, XuZ, YanZ, HeM, et al.Angiotensin-converting enzyme inhibitors provide a protective effect on hypoxia-induced injury in human coronary artery endothelial cells via Nrf2 signaling and PLVAP. Clinical Hemorheology and Microcirculation. 2024;87(2):141–70.
HuangC-H, YangM-L, TsaiC-H, LiY-C, LinY-J, KuanY-H. Ginkgo biloba leaves extract (EGb 761) attenuates lipopolysaccharide-induced acute lung injury via inhibition of oxidative stress and NF-κB-dependent matrix metalloproteinase-9 pathway. Phytomedicine. 2013;20(3-4):303–9.
8.
WangY, LiuM, WangH, BaiY, ZhangX, SunY, et al.Involvement of serotonin mechanism in methamphetamine-induced chronic pulmonary toxicity in rats. Human & Experimental Toxicology. 2013;32(7):736–46.
9.
MontesS, Juáfrez-RebollarD, Nava-RuízC, Sánchez-GarcíaA, Heras-RomeroY, RiosC, et al.Immunohistochemical study of Nrf2-antioxidant response element as indicator of oxidative stress induced by cadmium in developing rats. Oxidative Medicine and Cellular Longevity. 2015:570650.
10.
ChoiS. Encyclopedia of signaling molecules: SpringerNew York; 2012.
11.
AtiaAE, AbdullahAb. Modulation of Nrf2/Keap1 pathway by dietary phytochemicals. Int J Res Med Sci. 2014;2(2):375–81.
12.
D’OriaV, PetriniS, TravagliniL, PrioriC, PiermariniE, PetrilloS, et al.Frataxin deficiency leads to reduced expression and impaired translocation of NF-E2-related factor (Nrf2) in cultured motor neurons. International Journal of Molecular Sciences. 2013;14(4):7853–65.
13.
YiB, KasaiH, LeeH-S, KangY, ParkJY, YangM. Inhibition by wheat sprout (Triticum aestivum) juice of bisphenol A-induced oxidative stress in young women. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2011;724(1-2):64–8.
14.
Azizi JalilianF, KeshavarzG, KhazaeiS, NezamdoostM, HashemiSH, MamaniM, et al.The effects of nutrition bio-shield superfood powder on immune system function: A clinical trial study among patients with COVID-19. Frontiers in Immunology. 2022;13:919402.
15.
JungF, Krüger-GengeA, KöhlerS, MrowietzC, KüpperJ-H, BrauneS. Effects of Arthrospira platensis-derived phycocyanin on blood cells 1. Clinical Hemorheology & Microcirculation. 2023;85(3).
16.
BrauneS, Krüger-GengeA, KammererS, JungF, KüpperJ-H. Phycocyanin from Arthrospira platensis as potential anti-cancer drug: Review of in vitro and in vivo studies. Life. 2021;11(2):91.
17.
LiuRH. Dietary bioactive compounds and their health implications. Journal of Food Science. 2013;78(s1):A18–A25.
18.
DurantiM. Grain legume proteins and nutraceutical properties. Fitoterapia. 2006;77(2):67–82.
19.
MosadeghM, KhalkhaliA, ErfaniY, NezamdoostM. The effect of Nutrition Bio-shield superfood (NBS) on disease severity and laboratory biomarkers in patients with COVID-19: A randomized clinical trial. Microbial Pathogenesis. 2022;172:105792.
20.
PeterneljT-T, CoombesJS. Antioxidant supplementation during exercise training: Beneficial or detrimental?Sports Medicine. 2011;41:1043–69.
21.
AlsherifySM, HassanabadiA, ZerehdaranS, MoghaddamHN. The Effect of Herbal Product (NBS Superfood) Supplementation on Egg Quality Traits in Commercial Laying Hens. Poultry Science Journal. 2022;10(2):251–67.
22.
FardetA, CanletC, GottardiG, LyanB, LlorachR, RémésyC, et al.Whole-Grain and Refined Wheat Flours Show Distinct Metabolic Profiles in Rats as Assessed by a 1H NMR-Based Metabonomic Approach1. The Journal of Nutrition. 2007;137(4):923–9.
23.
ShahrabadiH, HaghighiAH, AskariR, Asadi-ShekaariM, SouzaDC, GentilP. Effect of high-intensity interval training on cardiac apoptosis markers in methamphetamine-dependent rats. Current Issues in Molecular Biology. 2022;44(7):3030–8.
24.
KimDY, JungSY, KimCJ, SungYH, KimJD. Treadmill exercise ameliorates apoptotic cell death in the retinas of diabetic rats. Molecular Medicine Reports. 2013;7(6):1745–50.
25.
YahyaeiF, BehradS, GhorbaniR, KhaksariM, AfrazES. The effects of berberine hydrochloride on the bone quality in methamphetamine-addicted rats after 3 weeks of withdrawal. Journal of Biological Studies. 2022;5(3 (Special Issue)):585.
26.
RenQ, MaM, YangC, ZhangJ, YaoW, HashimotoK. BDNF– TrkB signaling in the nucleus accumbens shell of mice has key role in methamphetamine withdrawal symptoms. Translational Psychiatry. 2015;5(10):e666.
27.
KordiN, AziziM, SamadiM, TahmasebiW. Can Methamphetamine-Induced Cardiotoxicity be Ameliorated by Aerobic Training and Nutrition Bio-shield Superfood Supplementation in Rats After Withdrawal?Cardiovascular Toxicology. 2024:1–13.
28.
ObernierJA, BaldwinRL. Establishing an appropriate period of acclimatization following transportation of laboratory animals. ILAR Journal. 2006;47(4):364–9.
29.
MattaL, FonsecaTS, FariaCC, Lima-JuniorNC, De OliveiraDF, MacielL, et al.The effect of acute aerobic exercise on redox homeostasis and mitochondrial function of rat white adipose tissue. Oxidative Medicine and Cellular Longevity. 2021;2021:4593496.
30.
PeiZ, YangC, GuoY, DongM, WangF. Effect of different exercise training intensities on age-related cardiac damage in male mice. Aging (Albany NY). 2021;13(17):21700.
31.
ChoH-Y, ReddySP, KleebergerSR. Nrf2 defends the lung from oxidative stress. Antioxidants & redox signaling. 2006;8(1-2):76–87.
32.
ChenL, RuQ, XiongQ, YangJ, XuG, WuY. Potential effects of Nrf2 in exercise intervention of neurotoxicity caused by methamphetamine oxidative stress. Oxidative Medicine and Cellular Longevity. 2022;2022:4445734.
33.
HeckerL, LogsdonNJ, KurundkarD, KurundkarA, BernardK, HockT, et al.Reversal of persistent fibrosis in aging by targeting Nox4-Nrf2 redox imbalance. Science Translational Medicine. 2014;6(231):231ra47.
34.
PapaiahgariS, KleebergerSR, ChoH-Y, KalvakolanuDV, ReddySP. NADPH oxidase and ERK signaling regulates hyperoxia-induced Nrf2-ARE transcriptional response in pulmonary epithelial cells. Journal of Biological Chemistry. 2004;279(40):42302–12.
35.
ChurchmanAT, AnwarAA, LiFY, SatoH, IshiiT, MannGE, et al.Transforming growth factor-β1 elicits Nrf2-mediated antioxidant responses in aortic smooth muscle cells. Journal of cellular and molecular medicine. 2009;13(8b):2282–92.
36.
LeeI-T, WangS-W, LeeC-W, ChangC-C, LinC-C, LuoS-F, et al.Lipoteichoic acid induces HO-1 expression via the TLR2/MyD88/c-Src/NADPH oxidase pathway and Nrf2 in human tracheal smooth muscle cells. The Journal of Immunology. 2008;181(7):5098–110.
37.
SekharKR, CrooksPA, SonarVN, FriedmanDB, ChanJY, MeredithMJ, et al.NADPH oxidase activity is essential for Keap1/Nrf2-mediated induction of GCLC in response to 2-indol-3-yl-methylenequinuclidin-3-ols. Cancer research. 2003;63(17):5636–45.
38.
KansanenE, KuosmanenSM, LeinonenH, LevonenA-L. The Keap1-Nrf2 pathway: Mechanisms of activation and dysregulation in cancer. Redox Biology. 2013;1(1):45–9.
39.
SpechtKS, KantS, AddingtonAK, McMillanRP, HulverMW, LearnardH, et al.Nox4 mediates skeletal muscle metabolic responses to exercise. Molecular metabolism. 2021;45:101160.
40.
HancockM, HafstadAD, NabeebaccusAA, CatibogN, LoganA, SmyrniasI, et al.Myocardial NADPH oxidase-4 regulates the physiological response to acute exercise. Elife. 2018;7:e41044.
41.
ZhouZ, YingC, ZhouX, ShiY, XuJ, ZhuY, et al.Aerobic exercise training alleviates renal injury in db/db mice through inhibiting Nox4-mediated NLRP3 inflammasome activation. Experimental Gerontology. 2022;168:111934.
42.
WangX, LiuM, ZhuMJ, ShiL, LiuL, ZhaoYL, et al.Resveratrol protects the integrity of alveolar epithelial barrier via SIRT1/PTEN/p-Akt pathway in methamphetamine-induced chronic lung injury. Cell Proliferation. 2020;53(3):e12773.
43.
ShiL, LiuB-Y, WangX, ZhuM-J, ChenL, ZhouM-Y, et al.RUNX3-dependent oxidative epithelial-to-mesenchymal transition in methamphetamine-induced chronic lung injury. Cell Stress and Chaperones. 2020;25(5):793–802.
44.
LiangL-Y, WangM-M, LiuM, ZhaoW, WangX, ShiL, et al.Chronic toxicity of methamphetamine: Oxidative remodeling of pulmonary arteries. Toxicology In Vitro. 2020;62:104668.
45.
Ciudad-MuleroM, DomínguezL, MoralesP, Fernández-RuizV, CámaraM. A Review of Foods of Plant Origin as Sources of Vitamins with Proven Activity in Oxidative Stress Prevention according to EFSA Scientific Evidence. Molecules. 2023;28(21):7269.
46.
HiguchiM. Antioxidant properties of wheat bran against oxidative stress. Wheat and rice in disease prevention and health: Elsevier; 2014. p. 181–99.
47.
KarabacakM, KanburM, EraslanG, SarıcaZS. The antioxidant effect of wheat germ oil on subchronic coumaphos exposure in mice. Ecotoxicology and environmental safety. 2011;74(7):2119–25.
48.
FanF, ZouY, FangY, LiP, XiaJ, ShenX, et al.Potential neuroprotection of wheat alkylresorcinols in hippocampal neurons via Nrf2/ARE pathway. Food & function. 2020;11(11):10161–9.
49.
BootsAW, DrentM, deBoer VC, BastA, HaenenGR. Quercetin reduces markers of oxidative stress and inflammation in sarcoidosis. Clinical nutrition. 2011;30(4):506–12.
50.
HeX, JarrellZR, LiangY, SmithMR, OrrML, MartsL, et al.Vanadium pentoxide induced oxidative stress and cellular senescence in human lung fibroblasts. Redox Biology. 2022;55:102409.
51.
Gutierrez-MariscalFM, Arenas-de LarrivaAP, Limia-PerezL, Romero-CabreraJL, Yubero-SerranoEM, López-MirandaJ. Coenzyme Q10 supplementation for the reduction of oxidative stress: Clinical implications in the treatment of chronic diseases. International journal of molecular sciences. 2020;21(21):7870.
52.
MasisiK, BetaT, MoghadasianMH. Antioxidant properties of diverse cereal grains: A review on in vitro and in vivo studies. Food chemistry. 2016;196:90–7.
