Abstract
We measured neutrophil filtrability at different temperatures and pH levels using a filtration method which provided a model system that is regarded to reflect conditions in the microcirculation. Neutrophil filtrability decreased as temperature fell (range: 40 to 4°C), but did not change with pH (range: 6.8 to 7.6). The decreased filtrability induced by cell cooling to 4°C was completely reversed by cell rewarming to 37°C and was also suppressed by the addition of cytochalasin B, which disrupts cellular actin filaments. Thus, neutrophil filtrability may be reduced by hypothermia, this reduction being, in part, to structural changes in cytoskeletal actin organization. These findings suggest that temperature setting is important in the measurement of neutrophil filtrability.
Get full access to this article
View all access options for this article.