Abstract
Erythrocyte Deformability (ED) was investigated by a Cell Transit-time Analyser (CTA) in three populations: elderly subjects, liver diseases patients and controls. Different conditions of measures were evaluated: anticoagulants (EDTA and heparin), buffers (PBS and tris), temperatures (25° C and 37° C), and different pressures.
The CTA analysed the deformability of individual cell passing through 5 um pores of a polycarbonate filter (Oligopore) under a low hydrostatic pressure. This method showed that E.D. was lower in elderly and in liver diseases groups than in the control group. Several fractions of less deformable red cells, as regard to their transit-times through the filter, were detected by the CTA. By contrast, red cells from controls group didn’t present these subpopulations.
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