Research on hemorheology is driven in part by its significance in blood diseases and the possible use of hemorheology as a diagnostic tool. However, existing data on blood rheology are limited largely to measurements of steady shear behavior often with varying measurement protocols and insufficient characterization of the physiology.
OBJECTIVE:
The effects of ex vivo aging and environmental conditions on blood viscosity are investigated to improve standards for hemorheology measurements.
METHODS:
Measurements on the viscosity of blood from nine healthy donors are obtained and the physiological state of the blood determined. Steady and transient shear measurements are reported as a function of time from withdrawal. The effect of transportation temperature is also assessed.
RESULTS:
Blood transported at 4 °C may exhibit anomalous viscosity variations for short to intermediate times, as opposed to blood transported at room temperature. A time of approximately 3.0 hours was identified as the maximum time after the initial test that accurate rheological tests can be conducted on blood samples.
CONCLUSIONS:
Measurement protocol and time limit guidelines are established for conducting accurate rheological measurements on blood.
KoenigW, SundM, FilipiakB, DoringA, LowelH, ErnstE. Plasma viscosity and the risk of coronary heart disease: Results from the MONICA-Augsburg cohort study, 1984 to 1992. Arterioscler Thromb Vasc Biol. 1998;18(5)768–72. doi:10.1161/01.ATV.18.5.768
2.
LoweG, RumleyA, NorrieJ, FordI, ShepherdJ, CobbeS, et al. Blood rheology, cardiovascular risk factors, and cardiovascular disease: The west of Scotland coronary prevention study. Thromb Haemost. 2000;84(4):553–8.
3.
Le DevehatC, VimeuxM, KhodabandehlouT. Blood rheology in patients with diabetes mellitus. Clin Hemorheol Microcirc. 2004;30(3-4):297–300.
4.
ConnesP, AlexyT, DetterichJ, RomanaM, Hardy-DessourcesMD, BallasSK. The role of blood rheology in sickle cell disease. Blood Rev. 2016;30(2):111–8. doi:10.1016/j.blre.2015.08.005
5.
ChienS, UsamiS, BertlesJF. Abnormal rheology of oxygenated blood in sickle cell anemia. J Clin Invest. 1970;49(4):623–34. doi:10.1172/JCI106273
6.
ShiYD, ArtmannG, AgostiR, LonghiniE. A modified Casson equation to characterize blood rheology for hypertension. Clin Hemorheol Microcirc. 1998;19(2):115–27.
7.
KwaanHC, BonguA. The hyperviscosity sydromes. Semin Thromb Hemost. 1999;25(2):199–208. doi:10.1055/s-2007-994921
CokeletGR, MerrillEW, GillilandER, ShinH, BrittenA, WellsRE. The rheology of human blood—measurement near and at zero shear rate. Trans Soc Rheol. 1963;7(1):303–17. doi:10.1122/1.548959
10.
DintenfassL. Thixotropy of blood and proneness to thrombus formation. Circ Res. 1962;11(2):233–9. doi:10.1161/01.RES.11.2.233
11.
ApostolidisAJ, BerisAN. Modeling of the blood rheology in steady-state shear flows. J Rheol. 2014;58(3):607–33. doi:10.1122/1.4866296
12.
CassonN. A flow equation for pigment-oil suspensions of the printing ink type. In: MillCC, editor. Rheology of Disperse Systems. New York: Pergamon Press; 1959, pp. 84–104.
13.
ApostolidisAJ, BerisAN. The effect of cholesterol and triglycerides on the steady state shear rheology of blood. Rheol Acta. 2016;55(6):497–509. doi:10.1007/s00397-015-0889-0
14.
BowenRAR, HortinGL, CsakoG, OtanezOH, RemaleyAT. Impact of blood collection devices on clinical chemistry assays. Clin Biochem. 2010;43(1-2):.4–25. doi:10.1016/j.clinbiochem.2009.10.001
15.
BrauneS, WalterM, SchulzeF, LendleinA, JungF. Changes in platelet morphology and function during 24 hours of storage. Clin Hemorheol Microcirc. 2014;58(1):159–70. doi:10.3233/CH-141876
16.
HoJ, SibbaldWJ, Chin-YeeIH. Effects of storage on efficacy of red cell transfusion: When is it not safe?Crit Care Med. 2003;31(12 Suppl):S687–97. doi:10.1097/01.CCM.0000099349.17094.A3
17.
HovavT, YedgarS, MannyN, BarshteinG. Alteration of red cell aggregability and shape during blood storage. Transfusion. 1999;39(3):277–81. doi:10.1046/j.1537-2995.1999.39399219284.x
18.
UyukluM, CengizM, UlkerP, HeverT, TripetteJ, ConnesP, et al. Effects of storage duration and temperature of human blood on red cell deformability and aggregation. Clin Hemorheol Microcirc. 2009;41(4):269–78. doi:10.3233/CH-2009-1178
19.
GoussetK, TsvetkovaNM, CroweJH, TablinF. Important role of raft aggregation in the signaling events of cold-induced platelet activation. Biochim Biophys Acta. 2004;1660(1-2):7–15. doi:10.1016/j.bbamem.2003.09.009
20.
PastoreJJ, FunakiM, JanmeyPA, BuckiR. Flavonoid-mediated inhibition of actin polymerization in cold-activated platelets. Platelets. 2005;16(6):362–7. doi:10.1080/09537100500124442
21.
NemethN, BaskurtOK, MeiselmanHJ, KissF, UyukluM, HeverT, et al. Storage of laboratory animal blood samples causes hemorheological alterations: Inter-species differences and the effects of duration and temperature. Korea-Aust Rheol J. 2009;21(2):127–33.
22.
BaskurtOK, BoynardM, CokeletGC, ConnesP, CookeBM, ForconiS, et al. New guidelines for hemorheological laboratory techniques. Clin Hemorheol Microcirc. 2009;42(2):75–97. doi:10.3233/CH-2009-1202
CokeletGR, BrownJR, CoddSL, SeymourJD. Magnetic resonance microscopy determined velocity and hematocrit distributions in a Couette viscometer. Biorheology. 2005;42(5):385–99.
25.
BureauM, HealyJC, BourgoinD, JolyM. Rheological hysteresis of blood at low shear rate. Biorheology. 1980;17(1-2):191–203.
26.
BullBS, ChienS, DormandyJA, KiesewetterH, LewisSM, LoweGDO, et al. Guidelines for measurement of blood viscosity and erythrocyte deformability. Clin Hemorheol Microcirc. 1986;6(5):439–53. doi:10.3233/CH-1986-6510
27.
ZhangJ, ZhangX, WangN, FanY, JuH, YangJ, et al. What is the maximum duration to perform the hemorheological measurement for human and mammals. Clin Hemorheol Microcirc. 2004;31(3):157–60.
28.
MewisJ, WagnerNJ. Colloidal suspension rheology. Cambridge: Cambridge University Press; 2012.
29.
MerrillEW, PelletierGA. Viscosity of human blood: Transition from Newtonian to non-Newtonian. J Appl Physiol. 1967;23(2):178–82.
30.
WilliamsDF, AskillIN, SmithR. Protein adsorption and desorption phenomena on clean metal surfaces. J Biomed Mater Res. 1985;19(3):313–20. doi:10.1002/jbm.820190312
31.
LeverettLB, HellumsJD, AlfreyCP, LynchEC. Red blood cell damage by shear stress. Biophys J. 1972;12(3):257–73. doi:10.1016/S0006-3495(72)86085-5
32.
BrookshierKA, TarbellJM. Evaluation of a transparent blood analog fluid: Aqueous xanthan gum/glycerin. Biorheology. 1993;30(2):107–16.
33.
MorenoL, CalderasF, Sanchez-OlivaresG, Medina-TorresL, Sanchez-SolisA, ManeroO. Effect of cholesterol and triglycerides levels on the rheological behavior of human blood. Korea-Aust Rheol J. 2015;27(1):1–10. doi:10.1007/s13367-015-0001-4
34.
DintenfassL. Internal viscosity of the red cell and a blood viscosity equation. Nature. 1968;219(5157):956–8. doi:10.1038/219956a0
35.
MerrillEW, GillilandER, LeeTS, SalzmanEW. Blood rheology: Effect of fibrinogen deduced by addition. Circ Res. 1966;18(4):437–46. doi:10.1161/01.RES.18.4.437
36.
OgunnaikeBA. Random phenomena: Fundamentals of probability and statistics for engineers. Boca Raton:CRC; 2009.
