Restricted accessResearch articleFirst published online 2017-5-5
Comparative erythrocyte deformability investigations by filtrometry,slit-flow and rotational ektacytometry in a long-term follow-up animal study on splenectomy and different spleen preserving operative techniques: Partial or subtotal spleen resection and spleen autotransplantation
BACKGROUND: Partial or subtotal spleen resection or spleen
autotransplantation can partly preserve/restore the splenic filtration function, as
previous studies demonstrated.
OBJECTIVE: For better evaluation and follow-up of the various
spleen-preserving operative techniques’ effectiveness versus splenectomy, a composite
methodological approach was applied in a canine experimental model.
METHODS: Beagle dogs were subjected to control (n = 6),
splenectomy (SE, n = 4), partial and subtotal spleen resection
(n = 4/each) or spleen autotransplantation groups (AU, Furka’s
spleen-chip method, n = 8). The follow-up period was 18 postoperative
(p.o.) months. Erythrocyte deformability was determined in parallel by bulk filtrometry
(Carat FT-1 filtrometer), slit-flow ektacytometry (RheoScan D-200) and rotational
ektacytometry (LoRRca MaxSis Osmoscan).
RESULTS: By filtrometry, relative cell transit time increased in the SE
group (mostly in animal Nr. SE-3), showing the highest values on the 3rd, 9th and in 18th
p.o. months. Elongation index values decreased in this group (both by slit-flow and
rotational ektacytometers). In general, AU and two resection groups’ values were lower
versus control and higher than in SE.
CONCLUSIONS: Forasmuch in the circulation both elongation by shear stress
and filtration occur, these various erythrocyte deformability testing methods together may
describe better the alterations. Considering the possible complications related to
functional asplenic-hyposplenic conditions, individual analysis of cases is highly
important.
BaskurtO.K., GelmontD. and MeiselmanH.J.,
Red blood cell deformability in sepsis, Am J
Respir Crit Care Med157(2) (1998),
421–427. doi: 10.1164/ajrccm.157.2.9611103
2.
BaskurtO.K., HardemanH.R., RamplingM.W., MeiselmanH.J., editors.,
Handbook of Hemorheology and Hemodynamics.
Amsterdam: IOS Press;
2007.
3.
BaskurtO.K., HardemanM.R., UyukluM., UlkerP., CengizM., NemethN., et
al., Parameterization of red blood cell elongation index – shear
stress curves obtained by ektacytometry, Scand J Clin Lab
Invest69(7) (2009),
777–788. doi: 10.3109/00365510903266069
4.
BaskurtO.K., HardemanM.R., UyukluM., UlkerP., CengizM., NemethN., et
al., Comparison of three commercially available ektacytometers
with different shearing geometries, Biorheology46(3) (2009),
251–264. doi: 10.3233/BIR-2009-0536
5.
BaskurtO.K.,
The role of spleen in suppressing the rheological alterations in
circulating blood, Clin Hemorheol Microcirc20(3) (1999),
181–188.
6.
BernatS.I., BogarL., CsornaiM., ImreS.,
JuricskayI., KollarL., NovakZ., PecsvaradyZs., PongraczE., RozsosI. and TothK.,
Módszertani útmutató a haemorheologiai mérések
végzéséhez, Érbetegségek1 (2005), 27–33.
[Article in Hungarian]
7.
CadiliA. and de
GaraC., Complications
of splenectomy, Am J Med121(5) (2008),
371–375. doi: 10.1016/j.amjmed.2008.02.014
8.
CrosbyW.H.,
Splenic remodeling of red cell surfaces,
Blood50(4) (1977),
643–645.
9.
FurkaI., MikoI.,
PappL., MikoT.,
Salvaging the spleen by experimental resection or
autotransplantation. In: Jubileuszowy Zjazd Towarzystwa
Chirurgow Polskich Vol. 2. Krakow,
1989, pp. 453–456.
10.
FurkaI., MikóI.,
MikóT. and PappL.,
Partial splenectomy performed by a special technique in
dogs, Acta Chir Hung31 (1990),
317–323.
11.
FurkaI., MikoI.,
SerfozoJ., FrendlI., HauckM.,
Autotransplantation of the spleen, In: Second
World Week of Professional Updating in Surgery and in Surgical and Oncological
Disciplines of the University of Milan, Lecture Book Vol. II.
Bologna: Monduzzi
Editore; 1990, pp.
767–769.
12.
FurkaI., HajduZ., SzendroiT., MikoI.,
BokkA., BarnakG.,
Spleen autotransplantation. Experimental and clinical
experiences. In: 23rd World Congress of the International
College of Surgeons, Cairo. Bologna:
Monduzzi Editore; 1992. pp.
907–912.
13.
FurkaI., MikoI.,
TothK., FurkaA., KappelmayerJ., SzikszaiZ., et
al., Haematological, hemorheological and catalase level changes
following splenectomy and spleen autotransplantation in experimental animals. Early
results, Acta Chir Austr29(Suppl.137) (1997),
31–32.
14.
GrandicL., PogorelicZ., BanovicJ., PerkoZ., BoschiV., IlicN.,
et al., Advantages of the spared surgical treatment of the
spleen injuries in the clinical conditions,
Hepatogastroenterology55 (2008),
2256–2258.
15.
HansenK. and SingerD.B.,
Asplenic-hyposplenic overwhelming sepsis: Postsplenectomy sepsis
revisited, Pediatr Dev Pathol4(2) (2001),
105–121.
16.
HarrisJ.W. and KellermeyerR.W.,
The Red Cell: Production, Metabolism, Destruction: Normal and Abnormal.
Revised Edition. Cambridge,
Massachusetts: Harvard University Press;
1970, p. 795.
17.
LipowskyH.H.,
Microvascular rheology and hemodynamics,
Microcirculation12(1) (2005),
5–15. doi: 10.1080/10739680590894966
18.
MikoI., FurkaI., SerfozoJ., JoosGy., TelekB., MateszK., et
al., Comparative study of haematological and micro-morphological
results in long-surviving spleen autotransplants, In: UranusS, ed.
Chirurgische Forschung, München, Bern, Wien, New
York, W. Zuckschwerdt Verlag;
1994, pp. 50–55.
19.
MikoI., BrathE., FurkaI., KovacsJ., KelvinD. and ZhongR.,
Spleen autotransplantation in mice: A novel experimental model for
immunology study, Microsurgery21(4) (2001),
140–142.
20.
MikoI., BrathE., NemethN., FurkaA., SipkaS.Jr,
PetoK., et
al., Spleen autotransplantation. Morphological and functional
follow-up after spleen autotransplantation in mice: A research summary,
Microsurgery27(4) (2007),
312–316. doi: 10.1002/micr.20362
21.
MikoI., NemethN., SajtosE., BrathE., PetoK.,
FurkaA., et
al., Splenic function and red blood cell deformability: The
beneficial effects of spleen autotransplantation in animal experiments,
Clin Hemorheol Microcirc45(2-4) (2010),
281–288. doi: 10.3233/CH-2010-1307
22.
MikoI., NemethN., SipkaS.Jr,
BrathE., PetoK.,
GulyasA., et
al., Hemorheological follow-up after splenectomy and spleen
autotransplantation in mice, Microsurgery26(1) (2006),
38–42. doi: 10.1002/micr.20208
23.
MooreE.E., CogbillT.H., JurkovichG.J., ShackfordS.R., MalangoniM.A. and
ChampionH.R.,
Organ injury scaling: Spleen and liver (1994 revision),
J Trauma38(3) (1995),
323–324.
24.
MorgensternL.,
Partial Splenectomy In: HiattJR, PhillipsEH, MorgensternL, eds.
Surgical Disease of the Spleen. Berlin,
Heidelberg: Springer-Verlag;
1997, pp. 263–279.
25.
PauloD.N.S. and
PauloM.S.L.,
Subtotal splenectomy with inferior pole preservation
(ESTPI), Salus J Health Sci1 (2015), 72–81. doi:
10.5935/2447-7826.201500009
26.
PetroianuA.,
Conservative Surgical Procedures on the Spleen, In:
PetroianuA, ed.
The Spleen, Sharjah,
Benthan Science Publishers Ltd; 2015, pp.
217–249.
27.
RobertsonD.A.,
SimpsonF.G. and
LosowskyM.S.,
Blood viscosity after splenectomy, Br Med J (Clin
Res Ed)283(6291) (1981),
573–575.
28.
SimchonS., JanK.M.
and ChienS.,
Influence of reduced red cell deformability on regional blood
flow, Am J Physiol253(4 Pt 2) (1987),
H898–H903.
29.
SzaboG., MikoI.,
SzikszaiZ., ImreS.
and FurkaI.,
The importance of red blood cells deformability measuring for following
the function of autotransplanted spleen chips, Acta Chir
Austr31(Suppl.159) (1999),
17–18.
30.
TrunkeyD.D., HulkaF., MullinsR.J.,
Splenic trauma. In: HiattJR, PhillipsEH, MorgensternL, eds.
Surgical Disease of the Spleen. Berlin,
Heidelberg: Springer-Verlag;
1997, pp. 233–261.
31.
WilliamB.M. and
CorazzaG.R.,
Hyposplenism: A comprehensive review. Part I: Basic concepts and
causes, Hematology12(1) (2007),
1–13. doi: 10.1080/10245330600938422
32.
WilliamB.M., ThawaniN., Sae-TiaS. and CorazzaG.R.,
Hyposplenism: A comprehensive review. Part II: Clinical manifestations,
diagnosis, and management, Hematology12(2) (2007),
89–98. doi: 10.1080/10245330600938463
33.
XuZ., ZhengY., WangX.,
ShehataN., WangZ.,
XieS. and SunY.,
Stiffering of sickle cell trait red blood cells under simulated strenous
exercise conditions, Microsystems & Nanoengineering2 (2016), 16061. doi: 10.1038/micronano.2016.61
