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Abstract

BACKGROUND

N6-methyladenosine (m⁶A) modification has been associated with non-small cell lung cancer (NSCLC) tumorigenesis.

OBJECTIVES

This study aimed to determine the functions of Vir-like m⁶A methyltransferase-associated (KIAA1429) and relaxin family peptide receptor 1 (RXFP1) in NSCLC.

METHODS

A quantitative real-time polymerase chain reaction was used to analyze the mRNA levels of KIAA1429 and RXFP1 in NSCLC. After silencing KIAA1429 or RXFP1 in NSCLC cells, changes in the malignant phenotypes of NSCLC cells were assessed using cell counting kit-8, colony formation, and transwell assays. Finally, the m⁶A modification of RXFP1 mediated by KIAA1429 was confirmed using luciferase, methylated RNA immunoprecipitation, and western blot assays.

RESULTS

KIAA1429 and RXFP1 were upregulated and downregulated in NSCLC, respectively. Silencing of KIAA1429 attenuated the viability, migration, and invasion of NSCLC cells, whereas silencing of RXFP1 showed the opposite function in NSCLC cells. Moreover, RXFP1 expression was inhibited by KIAA1429 via m⁶A-modification. Therefore, silencing RXFP1 reversed the inhibitory effect of KIAA1429 knockdown in NSCLC cells.

CONCLUSION

Our findings confirmed that the KIAA1429/RXFP1 axis promotes NSCLC tumorigenesis. This is the first study to reveal the inhibitory function of RXFP1 in NSCLC via KIAA1429-mediated m⁶A-modification. These findings may help identify new biomarkers for targeted NSCLC therapy.

Keywords

N6-methyladenosine KIAA1429 RXFP1 non-small cell lung cancer

1. Introduction

Lung cancer is a common malignancy with two subtypes: non-small cell lung cancer (NSCLC) and small cell lung cancer [1]. Among all lung cancers, NSCLC accounts for 85% [2]. Metastasis and chemoresistance are the two major obstacles to NSCLC therapy [3,4]. Targeted therapy is an emerging therapy that can prolong the overall survival of patients with NSCLC to a certain extent [5,6]. Therefore, investigating the key regulators of NSCLC development is crucial for improving NSCLC therapies.

N6-methyladenosine (m⁶A) is a post-transcriptional modification of RNAs that regulates RNA homeostasis [7]. Recently, increasing evidence has shown that abnormal m⁶A levels are correlated with tumorigenesis in NSCLC [8, 9, 10]. Vir-like m⁶A methyltransferase-associated (KIAA1429), a member of the m⁶A methyltransferase family, plays a key role in multiple human cancers [11, 12, 13]. For example, KIAA1429 upregulation in hepatocellular carcinoma can mediate the m⁶A methylation of GATA3 to promote the malignant phenotypes of hepatocellular carcinoma [14]. In colorectal cancer, high expression of KIAA1429 is associated with a poor prognosis and aggravates tumor growth [15]. In breast cancer, KIAA1429 is reported to be an oncogenic factor that regulates SMC1A mRNA stability [16] and CDK1 m⁶A modification [17]. Tang et al. confirmed that high expression of KIAA1429 in gefitinib-resistant NSCLC cells could enhance gefitinib resistance in NSCLC [18]. Here, this study aimed to deeply explore the regulatory mechanism of KIAA1429 in NSCLC.

Relaxin Family Peptide Receptor 1 (RXFP1), located on chromosome 4, is detected in multiple human tissues, including the lungs, heart, and ovaries [19]. The aberrant expression of RXFP1 is associated with cancer progression. For example, RXFP1, upregulated by the long noncoding RNA UCA1, promotes tumor growth in endometrial cancer [20]. Downregulation of RXFP1 in prostate cancer decreases metastasis and tumor growth [21]. The activation of RXFP1 by CTRP8 leads to the migration of glioblastoma cells [22]. However, the role of RXFP1 in NSCLC has not been elucidated.

This study aimed to verify the mechanism of action of KIAA1429 and RXFP1 in NSCLC progression and confirm their regulatory relationship between KIAA1429 and RXFP1 in NSCLC. The findings of our study may help identify new biomarkers for targeted NSCLC therapy.

2. Material and methods

2.1. Bioinformatic analysis

The online platform UALCAN (https://ualcan.path.uab.edu/index.html) [23] was used to analyze the levels of KIAA1429 and RXFP1 in TCGA-lung adenocarcinoma samples. GEPIA (http://gepia.cancer-pku.cn/index.html) [24] was used to analyze the correlation between KIAA1429 expression and the prognosis in lung adenocarcinoma. In addition, the correlation between KIAA1429 and RXFP1 expression in lung adenocarcinoma was analyzed using Pearson’s correlation analysis according to data from GEPIA.

2.2. Clinical samples

Table 1.
Clinical characteristics of the NSCLC patients.

Characteristics		No. (%)
Age, years	$ \leqslant $ 60	17 (42.5)
	$ \gt $ 60	23 (57.5)
Gender	Female	11 (27.5)
	Male	29 (72.5)
Tumor size, cm	$ \geqslant 3$	26 (65.0)
	$ \lt $ 3	14 (35.0)
Tumor differentiation	High	7 (17.5)
	Moderate	9 (22.5)
	Low	24 (60.0)
Lymph node metastasis	No	12 (30.0)
	Yes	28 (70.0)
TNM stage	I $ +$ II	15 (37.5)
	III $ +$ IV	25 (62.5)

NSCLC samples and paired adjacent normal samples were collected from 40 patients (average age, 60 years; 72.5% male) diagnosed with NSCLC at the Wuhan Third Hospital between July 1, 2021 and December 1, 2022. All patients enrolled in this study were initially diagnosed with NSCLC and did not receive any antitumor treatment. The ethics committee of Wuhan Third Hospital approved this study, and informed consent was obtained from all 40 patients. Table 1 shows patient characteristics.

Table 2.

List of primers used in this study.

Primer names	Sequences
KIAA1429 forward	5’-AGTGCCCCTGTTTTCGATAGG-3’
KIAA1429 reverse	5’-TACCAGCCTCTTAGCACCAG-3’
RXFP1 forward	5’-AAAAGAGATGATCCTTGCCAAACG-3’
RXFP1 reverse	5’-CCACCCAGATGAATGATGGAGC-3’
GAPDH forward	5’-GCACCGTCAAGGCTGAGAAC-3’
GAPDH reverse	5’-TGGTGAAGACGCCAGTGGA-3’

2.3. Cell culture

ProCell (China) provided all cell lines used in this study, including the human normal lung epithelial cell line BEAS-2B and two NSCLC cell lines (HCC827 and PC-9). All NSCLC cells were cultured in RPMI-1640 medium (ProCell) supplemented with 10% FBS (ProCell), whereas BEAS-2B cells were cultured in DMEM (ProCell) supplemented with 10% FBS. An incubator was used to incubate all cells at 37

$ ^{\circ }$

C and 5% CO

$ _{2}$

2.4. Quantitative real-time polymerase chain reaction (qRT-PCR)

Total RNA was isolated using a Total RNA Extraction Kit (R1200, Solarbio, China). Thereafter, 1

$ \mu $

g of total RNA was used to synthesize cDNA using the First Strand cDNA Synthesis Kit (4202, Wuhan Genenode Biotech Co., Ltd., China). Finally, the qRT-PCR was performed using SYBR Green qPCR Master Mix (HY-K0501, MedChemExpress, China) at 95

$ ^{\circ }$

C for 5 min, and 40 cycles at 95

$ ^{\circ }$

C for 5 s and 60

$ ^{\circ }$

C for 30 s. 2-^ΔΔCt approach was used to calculate the relative expression of mRNA with the normalization of GAPDH. All primer sequences are listed in Table 2.

2.5. Cell transfection

Two small interfering RNAs (siRNAs) targeting KIAA1429 (si-KIAA1429#1 and si-KIAA1429#2) and RXFP1 (si-RXFP1), negative control (si-NC), KIAA1429 overexpression vector (OE-KIAA1429), RXFP1 overexpression vector (OE-RXFP1), and negative control empty vector (OE-NC) were purchased from RiboBio (China). NSCLC cells were transfected with the above-mentioned vectors using Lipo6000 (Beyotime, China).

2.6. Cell counting kit-8 (CCK8) assay

NSCLC cell viability was assessed using the Cell Counting Kit-8 (CCK8; Beyotime, China). Cell suspension (100

$ \mu $

l/well) containing 1000 NSCLC cells was added to a 96-well plate. After transfecting NSCLC cells at 0, 24, 48, and 48 h, CCK8 solution (10

$ \mu $

l/well) was added to a 96-well plate and incubated for 2 h. Finally, the optical density (OD) at 450 nm was measured using an HM-SY96S microplate reader (Hengmei, China).

2.7. Colony formation assay

A total of 500 transfected NSCLC cells were added to a 6-well plate. After incubation for 2 weeks, the washed NSCLC cells were fixed with 4% paraformaldehyde and stained with crystal violet. Images of the colonies were captured using a digital camera (Nikon, Japan) to calculate the number of colonies formed.

2.8. Transwell migration and invasion assays

Cell suspension (200

$ \mu $

l) without FBS containing transfected NSCLC cells (5

$ \times $

10⁴ cells/ml) was added to the upper chamber coated with (for invasion) or without (for migration) Matrigel, whereas 10% FBS mixed with medium was added into the lower chamber. After cultivating for 24 h, the residual cells on the upper layer were removed using cotton swabs, migrated or invasive cells were fixed and stained, and counted using a light microscope (Olympus, Japan).

Fig. 1.

KIAA1429 expression in non-small cell lung cancer (NSCLC). (A) UALCAN was used to analyze KIAA1429 expression in TCGA-lung adenocarcinoma samples. (B) GEPIA was used to analyze the correlation between KIAA1429 expression and prognosis in TCGA-lung adenocarcinoma samples. (C) Quantitative real-time polymerase chain reaction (qRT-PCR) analysis confirmed KIAA1429 expression in 40 NSCLC and paired normal tissues collected in this study. A

$ t$

-test was used to compare the significant difference in KIAA1429 expression between normal and tumor tissues. (D) The KIAA1429 expression in different TNM stages was analyzed using qRT-PCR. A

$ t$

-test was used to compare the significant difference in KIAA1429 expression in the I

$ +$

II and III

$ +$

IV stages. (E) qRT-PCR analysis confirmed KIAA1429 expression in two NSCLC cell lines (HCC827 and PC-9) and the human normal lung epithelial cell line BEAS-2B. ^**P < 0.001 vs. BEAS-2B calculated using the analysis of variance indicated a significant difference in KIAA1429 expression in NSCLC cells and human normal lung epithelial cells. Error bars represent the standard deviation.

2.9. Luciferase assay

The wild-type RXFP1 vector (RXFP1-WT) with m⁶A modified sites and the mutant RXFP1 vector (RXFP1-MUT) without m⁶A modified sites were constructed by RiboBio (China) using the pGL3 vector (Promega, USA). HCC827 and PC-9 cells were seeded in a 24-well plate and incubated until 70% confluence was reached. The RXFP1-WT/RXFP1-MUT vectors were transfected into HCC827 and PC-9 cells with si-NC/si-KIAA1429 using Lipo6000 (Beyotime, China). After 24 h, the luciferase activity was detected using the Firefly Luciferase Reporter Gene Assay Kit (RG005, Beyotime).

2.10. Methylated RNA immunoprecipitation (MeRIP) assay

This assay was performed using the MeRIP m⁶A Transcriptome Profiling Kit (RiboBio, China). HCC827 and PC-9 cells were transfected with si-NC or si-KIAA1429 and collected to isolate total RNA. The isolated total RNA was fragmented by adding 2

$ \mu $

l of 10

$ \times $

fragmentation buffer. Thereafter, 300

$ \mu $

g of fragmented total RNA was incubated using 500

$ \mu $

l MeRIP reaction mixture and 25

$ \mu $

l magnetic beads pre-combined with 10

$ \mu $

g m6A antibody or IgG antibody (negative control) for 2 h at 4

$ ^{\circ }$

C. Finally, the beads were washed twice with elution buffer, and the eluted RNA was subjected to qRT-PCR to detect RNA enrichment.

2.11. Western blotting

Proteins were isolated from the cells using RIPA buffer (Beyotime, China). After detecting protein concentration using the BCA method, 30

$ \mu $

g protein loaded onto 10% SDS-PAGE were blotted onto PVDF membranes. The membranes were blocked using 5% skimmed milk. Thereafter, the membranes were incubated with anti-KIAA1429 (abs152580, Absin, China), anti-RXFP1 (abs139785, Absin), and anti-GAPDH (abs132004, Absin) antibodies overnight at 4^∘C. Further, rabbit secondary antibody (A0208, Beyotime) was added to the membranes and incubated for 1 h at 22^∘C. Finally, the protein bands were visualized using BeyoECL Plus (P0018S; Beyotime).

2.12. Data analysis

GraphPad Prism software 8.0.1 was used for data analysis. A

$ t$

-test was used to compare the differences between the two groups. The analysis of variance was used to compare the differences among multiple groups. All data from three independent experiments were shown as the mean

$ \pm $

standard deviation.

$ P\lt $

0.05 was set as the statistical difference.

3. Results

3.1. KIAA1429 is highly expressed in NSCLC

According to data from TCGA-lung adenocarcinoma samples, KIAA1429 was upregulated in 515 lung adenocarcinoma samples (Fig. 1A), and patients with high KIAA1429 expression had poorer prognoses (Fig. 1B). We collected 40 NSCLC tissue samples and paired them with normal tissue samples to further explore KIAA1429 expression. qRT-PCR analysis revealed that KIAA1429 was upregulated in NSCLC tissues (Fig. 1C) and that KIAA1429 expression was enhanced in TNM stages III

$ +$

IV (Fig. 1D). Similar results were observed at the cellular level; high expression of KIAA1429 was observed in NSCLC cells (Fig. 1E). These findings confirm the upregulation of KIAA1429 in NSCLC.

3.2. Downregulating KIAA1429 attenuated NSCLC cell malignancy

Fig. 2.

The effect of KIAA1429 knockdown on non-small cell lung cancer (NSCLC) cell malignancy. Quantitative real-time polymerase chain reaction (A) and western blotting (B) confirmed the transfection efficiency of two siRNAs targeting KIAA1429 (si-KIAA1429#1 and si-KIAA1429#2) in NSCLC cells. (C) The cell counting kit-8 assay was used to analyze the effect of KIAA1429 knockdown on NSCLC cell viability. (D) A colony formation assay was used to determine the effect of KIAA1429 knockdown on NSCLC cell proliferation. Transwell migration (E) and invasion (F) assays were used to evaluate the effect of KIAA1429 knockdown on the migration and invasion abilities of NSCLC cells. ^**P < 0.001 vs. si-NC calculated using the analysis of variance confirmed the significant differences in NSCLC cell function among the si-NC, si-KIAA1429#1, and si-KIAA1429#2 groups. All data is representative of three independent experiments. Error bars represent the standard deviation.

To determine the function of KIAA1429 in NSCLC, two siRNAs targeting KIAA1429 (si-KIAA1429#1 and si-KIAA1429#2) were transfected into NSCLC cells to downregulate KIAA1429 expression. qRT-PCR and western blotting analyses confirmed that both si-KIAA1429#1 and si-KIAA1429#2 decreased KIAA1429 expression in NSCLC cells (Figs 2A and 2B). Knocking down KIAA1429 reduced the viability of NSCLC cells, as evidenced by the OD values detected by the CCK8 assay (Fig. 2B). The colony formation assay further demonstrated that cell proliferation was inhibited by si-KIAA1429#1 or si-KIAA1429#2 in NSCLC cells (Fig. 2C). Transwell migration and invasion assays revealed that cell migration and invasion abilities were impaired when NSCLC cells were transfected with si-KIAA1429#1 or si-KIAA1429#2 (Figs 2D and 2E). These results confirm that KIAA1429 knockdown suppresses tumorigenesis in NSCLC cells.

3.3. KIAA1429-mediated m⁶A modification of RXFP1 in NSCLC cells

Fig. 3.

KIAA1429-mediated N6-methyladenosine (m⁶A) modification of relaxin family peptide receptor 1 (RXFP1) in non-small cell lung cancer (NSCLC) cells. (A) UALCAN was used to analyze RXFP1 expression in TCGA-lung adenocarcinoma samples. (B) GEPIA was used to analyze the correlation between KIAA1429 and RXFP1 expression in TCGA-lung adenocarcinoma samples. (C) Quantitative real-time polymerase chain reaction confirmed RXFP1 expression in 40 NSCLC and paired normal tissues collected in this study. A

$ t$

-test was used to compare the significant difference in RXFP1 expression between normal and tumor tissues. (D) Pearson’s correlation analysis was used to determine the correlation between RXFP1 and KIAA1429 expression in 40 NSCLC samples.

$ R=$

$ -$

0.6574 and

$ P\lt $

0.0001 indicated a significant negative correlation. (E) The m⁶A modification site of RXFP1 was predicted using RBMase v2.0. (F) Luciferase activity in NSCLC cells co-transfected with RXFP1-WT/RXFP1-MUT and si-NC/si-KIAA1429 was quantified using a luciferase assay. ^*P < 0.05, ^**P < 0.001 vs. RXFP1-WT

$ +$

si-NC calculated using the analysis of variance confirmed the significant difference in luciferase activities among different groups. (G) MeRIP assay was used to detect the m⁶A levels of RXFP1 in NSCLC cells transfected with si-KIAA1429. ^**P < 0.001 calculated using the analysis of variance verified the significant difference in RXFP1 m⁶A levels among different groups. qRT-PCR (H) and western blotting (I) confirmed the levels of RXFP1 mRNA and protein in NSCLC cells transfected with OE-KIAA1429 or si-KIAA1429. ^**P < 0.001 vs. OE-NC calculated using a

$ t$

-test showed the significant difference in RXFP1 expression between the OE-NC and OE-KIAA1429 groups. ^##P < 0.001 vs. si-NC calculated using a

$ t$

-test showed a significant difference in RXFP1 expression between the si-NC and si-KIAA1429 groups. Error bars represent the standard deviation.

By analyzing data from TCGA lung adenocarcinoma samples, we found that RXFP1 was downregulated in tumor samples (Fig. 3A), and its expression in lung adenocarcinoma samples was negatively correlated with KIAA1429 expression (Fig. 3B). In our clinical samples, RXFP1 expression was reduced by approximately 50% in NSCLC samples compared with normal samples (Fig. 3C). Pearson correlation analysis further indicated a negative correlation between RXFP1 and KIAA1429 expression in NSCLC samples (

$ R=$

$ -$

0.6574; Fig. 3D). RMBase v2.0 predicted the m⁶A modification site of RXFP1 (Fig. 3E). We constructed RXFP1-WT and RXFP1-MUT vectors according to the predicted m⁶A modification site to perform a luciferase assay. The results showed that si-KIAA1429 enhanced the luciferase activity of the RXFP1-WT group but did not affect the luciferase activity of the RXFP1-MUT group (Fig. 3F), indicating that KIAA1429 mediated m⁶A modification of RXFP1. The MeRIP assay further revealed that the m⁶A levels of RXFP1 were reduced by si-KIAA1429 in NSCLC cells (Fig. 3G). Finally, qRT-PCR and western blotting revealed that both RXFP1 mRNA and protein levels were reduced in KIAA1429 overexpressed NSCLC cells and enhanced in KIAA1429 knockdown NSCLC cells (Figs 3H and 3I). These findings indicated that KIAA1429 mediates RXFP1 m⁶A modification to regulate RXFP1 expression.

3.4. KIAA1429 knockdown reversed the promotive effects of RXFP1 knockdown on NSCLC cell malignancy

Fig. 4.

KIAA1429 knockdown reversed the effects of relaxin family peptide receptor 1 (RXFP1) knockdown on non-small cell lung cancer (NSCLC) cell malignancy. (A) The cell counting kit-8 assay was used to analyze the changes in the viability of transfected NSCLC cells. (B) A colony formation assay was used to assess the changes in proliferation of transfected NSCLC cells. Transwell migration (C) and invasion (D) assays were used to evaluate the changes in migration and invasion abilities of transfected NSCLC cells. ^**P < 0.001 vs. si-NC. ^##P < 0.001 vs. si-KIAA1429

$ +$

si-RXFP1 calculated using the analysis of variance indicated the significant difference in cell function among different groups. Error bars represent the standard deviation.

The effects of the interaction between KIAA1429 and RXFP1 on NSCLC cells were verified using CCK8, colony formation, and transwell migration/invasion assays. CCK8 and colony formation assays showed that si-RXFP1 increased NSCLC cell viability and proliferation (Figs 4A and 4B). The migration and invasion abilities of NSCLC cells were enhanced by transfecting with si-RXFP1 (Figs 4C and 4D). However, si-KIAA1429 mitigated the increase in viability, proliferation, migration, and invasion of NSCLC cells induced by si-RXFP1 (Figs 4A–4D). All the data showed that RXFP1 knockdown enhanced NSCLC cell malignancy; however, this effect was relieved by KIAA1429 knockdown.

3.5. KIAA1429 overexpression reversed the inhibitory effects of RXFP1 overexpression on NSCLC cell malignancy

Fig. 5.

KIAA1429 overexpression reversed the inhibitory effects of relaxin family peptide receptor 1 (RXFP1) overexpression on non-small cell lung cancer (NSCLC) cell malignancy. (A) The cell counting kit-8 assay was used to analyze the changes in viability of transfected NSCLC cells. (B) A colony formation assay was used to assess the changes in proliferation of transfected NSCLC cells. Transwell migration (C) and invasion (D) assays were used to evaluate the changes in migration and invasion abilities of transfected NSCLC cells. ^**P < 0.001 vs. OE-NC. ^##P< 0.001 vs. OE-KIAA1429

$ +$

OE-RXFP1 calculated using the analysis of variance indicated the significant difference in cell function among different groups. Error bars represent the standard deviation.

We further explored the effects of KIAA1429 and RXFP1 overexpression on NSCLC cells using cell function assays. After detecting cell viability and proliferation using CCK8 and colony formation assays, we observed that KIAA1429 overexpression increased viability and proliferation of NSCLC cells, whereas RXFP1 overexpression decreased viability and proliferation of NSCLC cells (Figs 5A and 5B). Meanwhile, the transwell assay confirmed that the migration and invasion abilities of NSCLC cells were enhanced by KIAA1429 overexpression, whereas RXFP1 overexpression impaired the migration and invasion abilities of NSCLC cells (Figs 5C and 5D). In addition, KIAA1429 overexpression relieved the inhibitory effects of RXFP1 overexpression on the viability, proliferation, migration, and invasion of NSCLC cells (Figs 5A–5D). These results indicate that the inhibitory effects of RXFP1 overexpression on NSCLC cell malignancy could be reversed by KIAA1429 overexpression.

4. Discussion

The m⁶A modification has been confirmed as a key regulatory mechanism in NSCLC carcinogenesis [8, 9, 25, 26]. In this study, we observed upregulation of KIAA1429 and downregulation of RXFP1 in NSCLC. Using cell function experiments, we confirmed that silencing KIAA1429 suppressed NSCLC cell malignancy, whereas silencing RXFP1 enhanced NSCLC cell malignancy. In addition, KIAA1429 mediates m⁶A modification of RXFP1 to inhibit RXFP1 expression, thereby playing an oncogenic role in NSCLC.

Increasing evidence has revealed the regulatory functions of m⁶A modifications in tumorigenesis [27, 28, 29]. KIAA1429, an m⁶A methyltransferase complex, plays an essential role in tumorigenesis by recruiting METTL3/METTL14 or mediating mRNA methylation [13, 30]. Some studies have demonstrated the promoting role of KIAA1429 in lung cancer tumorigenesis. For example, high expression of KIAA1429 has been correlated with a poor prognosis of lung adenocarcinoma, and KIAA1429 induces tumor growth and metastasis of lung adenocarcinoma by regulating BTG2 m⁶A modification [31]. KIAA1429, also called VIRMA, facilitates tumor growth in NSCLC via m⁶A-dependent degradation of DAPK3 mRNA [32]. Consistent with previous studies, this study confirmed the upregulation of KIAA1429 in NSCLC and the promoting effect of KIAA1429 on NSCLC tumorigenesis. According to previous studies, KIAA1429 enhances the malignancy of lung adenocarcinoma by regulating MUC3A m⁶A [33] or BTG2 m⁶A modifications [31]. These studies suggest that KIAA1429 regulates the genes’ m⁶A modification to induce lung cancer progression. Therefore, we analyzed the regulatory mechanism of KIAA1429 in NSCLC. Interestingly, we confirmed that KIAA1429-induced RXFP1 m⁶A modification contributes to NSCLC cell malignancy, which is different from a previous study showing DAPK3 m⁶A modification [32]. Therefore, our study is the first to identify a new gene, RXFP1, that could be regulated by KIAA1429-mediated m⁶A-modification in NSCLC.

RXFP1, a relaxin receptor, has been reported to act as a key regulator of tumorigenesis [21, 22, 34]. In prostate cancer, RXFP1 downregulation could effectively reduce the metastasis rate in vivo [21]. CTRP8-induced RXFP1 activation contributes to glioblastoma cell migration [22]. RXFP1 regulation by UCA1 has also been shown to facilitate the development of endometrial cancer [20]. Previous studies have suggested an oncogenic function of RXFP1 in cancer; however, its function in NSCLC has not yet been revealed. Our study showed that RXFP1 was downregulated in NSCLC. Moreover, our results support the conclusion of the study by Xie et al. that patients with lung adenocarcinoma with overexpressed RXFP1 had a long overall survival rate, suggesting an inhibitory effect of RXFP1 overexpression in lung adenocarcinoma [35]. Owing to the presence of lung adenocarcinoma in NSCLC, we suspected that RXFP1 might be an antitumor gene in NSCLC. Based on cell functional experiments, we found that RXFP1 knockdown in NSCLC cells enhanced malignancy. Moreover, RXFP1 expression in NSCLC cells was regulated by KIAA1429-mediated m⁶A-modification. This is the first study to clarify the function and mechanism of action of RXFP1 in NSCLC.

According to previous reports, KIAA1429 accelerates gefitinib resistance in NSCLC [18] and lung adenocarcinoma cells [36], suggesting that a drug targeting KIAA1429 may improve the therapeutic effect of gefitinib resistance in patients with NSCLC in clinical settings. Meanwhile, in lung adenocarcinoma, high expression of KIAA1429 indicated a larger tumor size, higher affinity to the lymph nodes, distant metastasis, and a lower overall survival rate [33], whereas high expression of RXFP1 indicated a higher overall survival rate [35]. This suggests that KIAA1429 and RXFP1 may serve as biomarkers for NSCLC diagnosis at early stages or prognosis in clinical settings.

The present study confirmed the functions of KIAA1429 and RXFP1 in NSCLC. However, this study has some limitations that warrant further investigation. First, the regulatory mechanism of NSCLC in vivo is complex, and whether KIAA1429-mediated RXFP1 m⁶A modification has an inhibitory effect on tumor growth in vivo needs to be explored in the future. Second, KIAA1429 was reported to accelerate gefitinib resistance in NSCLC [18]; however, whether regulating RXFP1 could reverse the effect of KIAA1429 on gefitinib resistance in NSCLC require further investigation.

5. Conclusion

Overall, this study revealed that RXFP1 attenuated NSCLC tumorigenesis by regulating NSCLC cell malignancy via KIAA1429-mediated m⁶A modification. Our findings may help identify potential biomarkers for targeted NSCLC therapy.

Footnotes

Acknowledgments

None.

ORCID iDs

Zhixiang Zhang

Jipeng Guo

Chongwen Gong

Sai Wu

Sun Yanlei

Conflict of interest

The authors declare that there were no conflicts of interest.

Ethics approval

The Ethics Committee of Wuhan Third Hospital (Wuhan, China) approved this study. Clinical tissues specimen processing was accomplished in accordance with the ethical principles of the Declaration of Helsinki. All patients completed an informed consent form.

Consent to participate

All patients signed a written informed consent.

Consent for publication

All authors have provided their consent for the publication of this work.

Data availability statement

The data used to support the findings of this study are included within the article.

Authors’ contributions

Conception: ZXZ, JPG, and CWG.

Interpretation or analysis of data: ZXZ, JPG, CWG, and SW.

Preparation of the manuscript: ZXZ, JPG, and CWG.

Revision for important intellectual content: YLS.

Supervision: YLS.

All authors approved this article.

Funding

None.

Supplementary data

The supplementary files are available to download from .

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KIAA1429-mediated RXFP1 attenuates non-small cell lung cancer tumorigenesis via N6-methyladenosine modification

Abstract

BACKGROUND

OBJECTIVES

METHODS

RESULTS

CONCLUSION

Keywords

1. Introduction

2. Material and methods

2.1. Bioinformatic analysis

2.2. Clinical samples

Table 1. Clinical characteristics of the NSCLC patients.

2.4. Quantitative real-time polymerase chain reaction (qRT-PCR)

2.5. Cell transfection

2.6. Cell counting kit-8 (CCK8) assay

2.7. Colony formation assay

2.8. Transwell migration and invasion assays

2.10. Methylated RNA immunoprecipitation (MeRIP) assay

2.11. Western blotting

2.12. Data analysis

3. Results

3.1. KIAA1429 is highly expressed in NSCLC

3.2. Downregulating KIAA1429 attenuated NSCLC cell malignancy

5. Conclusion

Footnotes

Acknowledgments

ORCID iDs

Conflict of interest

Ethics approval

Consent to participate

Consent for publication

Data availability statement

Authors’ contributions

Funding

Supplementary data

References

Table 1.
Clinical characteristics of the NSCLC patients.