Upregulated lncRNA-PCAT1 is closely related to clinical diagnosis of multiple myeloma as a predictive biomarker in serum

Abstract

OBJECTIVE:

The purpose of this study was to explore serum PCAT-1 expression in multiple myeloma (MM) and examine the potential usefulness of this molecule as a biomarker for diagnosis in MM.

METHODS:

Serum samples were collected from 60 newly diagnosed untreated MM patients, and 48 healthy controls. Serum PCAT-1 expression levels were detected by RT-qPCR. In addition, correlations between the relative expression of serum PCAT-1 and the concentrations of lactic acid dehydrogenase (LDH), β₂M, λ light chain and κ light chain were assessed.

RESULTS:

It was found that the relative expression of serum PCAT-1 in MM patients (81.02 ± 136.9) was higher than that in healthy controls(3.17 ± 5.75) (U= 307.0, P< 0.0001) and was significantly correlated withβ₂M concentration (r= 0.461, P= 0.0002), but not with LDH,κ light and λ light chain concentration (r= 0.061, P= 0.641; r= 0.007, P= 0.956; r=-0.090, P= 0.499 respectively). Additionally, it was significantly correlated with different isotype of MM(H= 7.464, P= 0.024). The AUC of the ROC curve of serum PCAT-1 was 0.892(95% CI 0.833-0.950), which was higher than other markers. Combining PCAT-1 and β₂M together, the sensitivity was highest compared with other markers alone, or combined.

CONCLUSION:

The expression levels of serum PCAT-1 in MM patients were significantly higher than that in healthy controls, suggesting that it may be useful in the auxiliary diagnosis of MM.