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Abstract

In this work, Fourier transform infrared (FT-IR) spectra of untreated and microwaves irradiated genomic DNAs extracted from leaves of different grapevine varieties, respectively, have been analyzed between 1800–800 cm⁻¹, in order to investigate their screening characteristic features and their structural response to microwaves treatment at 869–894 MHz frequency band. FT-IR vibrational modes for each of these cases, spectroscopic band assignments and structural interpretations of genomic DNAs are reported. No significant microwaves damage, concerning DNA bases structure, base pairing and base unstacking was found. Conformational changes of specific DNA segments and effects on deoxyribose seem to appear after microwaves irradiation. Grapevine varieties dependent microwaves influence on the structure of DNA has been found. Best nucleic acids structural tolerance upon microwave irradiation, as compared with the non-exposed DNA has been found in the case of genomic DNA isolated from ‘Chasselas Doré’ grapevine variety.

Keywords

Genomic DNA grapevine varieties FT-IR spectroscopy microwaves irradiation

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Structural response of genomic DNA from grapevine ( Vitis vinifera L.) varieties to microwaves irradiation: A Fourier transform infrared spectroscopy assessment

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