Effect of Ca ++ on the structure and rheology of canine tracheal mucin

Abstract

Mucin glycoproteins are known to be the principal determinants of epithelial mucus rheology and hence of mucociliary transport rates. We are studying the structure of such glycoproteins using a model mucin purified from canine tracheal pouch secretions. Of particular interest is the effect on mucin structure of increased Ca⁺⁺ such as occurs in certain disease states. Quasielastic laser light scattering was used to study the effect of Ca⁺⁺ on the hydrodynamic radius of the mucin molecules. Scattering data from 0.3 mg/ml mucin solutions in physiological phosphate buffer containing 0,5 × 10⁻⁵ M, and 5 × 10⁻⁴ M Ca⁺⁺ were analyzed to obtain an average translational diffusion coefficient and the distribution of molecular radii for the dispersion. The effect of Ca⁺⁺ was to decrease the average Stokes radius. The light scattering results are supported by rheologic measures of mucin gel viscoelasticity.