Abstract
Summary
1. Specimens of urine from mumps patients were concentrated by ultra-centrifugation prior to tissue culture inoculation. This concentration resulted in 3 times as many mumps virus isolations as simultaneously tested portions of the same urine prepared by simple dilution. 2. All isolations were made in primary monkey kidney cell cultures; only 2 were made also in simultaneously inoculated HeLa cultures and none were made in HeLa cultures only. 3. Mumps virus was recovered from the urine of each of 13 patients within the first 14 days after onset of salivary gland pain or swelling. Virus was isolated from urine as early as the first and as late as the 14th day of illness. No testing was done beyond the 20th day.
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