Abstract
Summary and conclusions
Fibrinogen and other plasma proteins are apparently adsorbed on the hydrophilic surface of normal leukocytes, thereby complicating the interpretation of leuko-agglutinin tests. Normal leukocytes separated from whole blood (but not leukocytes separated from defibrinated blood) were rapidly and non-specifically agglutinated when suspended in normal untreated serum. Polymerization of adsorbed fibrinogen appeared to be the mechanism of such non-specific agglutination, which could be prevented by adequate decalcification of the suspending serum. Addition of EDTA to all test sera has permitted construction of a satisfactory leuko-agglutinin test using white cells separated from whole blood.
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