Abstract
Summary
1. Fasted rabbits and rats were used as assay animals for Hemopoietine preparations. Depressed erythropoiesis manifests itself by low plasma iron turnover and uptake of Fe59 in erythrocytes in rabbits, and by low erythrocyte and high liver Fe59 in rats. 2. Injections of extracts of boiled plasma from phenylhydrazine-treated rabbits increases plasma iron turnover and Fe59 uptake by erythrocytes in rabbits, and produces an increase in erythrocyte Fe59, accompanied by a drop of liver Fe59 in rats. Uptake of Fe59 in erythrocytes is proportional to log dose of Hemopoietine administered, while Fe59 erythrocyte/liver ratio is proportional to dose. Active fractions have been prepared from both urine and plasma of anemic rabbits by alcohol fractionation. The active material is non-dialyzable and contains more than 10 mg carbohydrate per 100 mg protein.
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