Superiority of Fluorescein Isothiocyanate (Riggs) for Fluorescent-Antibody Technic with a Modification of its Application

Abstract

Summary

1) The globulin fractions of antisera representing bacterial, viral, mycotic agents and antiglobulin fractions were labeled with 2 derivatives of fluorescein amine by 3 methods. 2) Fluorescein isothiocyanate was shown to be superior in stability, ease of conjugation, and degree of fluorescence. This direct method of adding the dye to a dilute buffered antiserum eliminates the need for organic reagents which may denature the protein.