Abstract
Summary
Addition of human plasma to washed homologous erythrocytes pretreated with sodium oleate results in hemolysis. Whole plasma is a less efficient lytic agent than is the homologous albumin or globulin moieties prepared by ammonium sulfate fractionation. Saturation of fatty acid binding capacity of plasma or plasma protein fractions, by addition of exogenous oleate inhibits lytic activity. Hemolysis appears related to acceleration of lytic activity of oleate molecules fixed to the surface of the red blood cell following binding by plasma constituents. Similar results were obtained with other fatty acids and with erythrocyte-plasma systems from other mammalian species.
Get full access to this article
View all access options for this article.