Method for Studying Translocation of Some Radioisotopes into Organs from Site of Injection. ∗

Abstract

Summary and conclusions

(1) Doses of 0.05 mc of Y⁹⁰Cl³ solution and of CrP³²O⁴ colloidal suspension in 0.05 or 0.1 ml were injected into ventral surface of tails in mice. At intervals of 1 minute to 3 days after injection, tails were serially amputated and preserved until 3rd day when every mouse was sacrificed; their organs (liver, lung, spleen, kidney), tails and heart blood (0.3 ml) were emulsified, dried and tested for radioactivity content with a Geiger Tube Detector. (2) Results showed only a narrow range of radioactivity for each organ specimen calculated as a product of the average blood radioactivity in the same group of mice (amputated at the same time). (3) The calculated and tabulated data show that a significant proportion of CrP³²O⁴ was translocated into the liver from the tail; for Y⁹⁰ the initial storage in the tail was very high, but some translocation occurred, presumably by excretion. (4) The pattern of Y⁹⁰ and CrP³²O₄ distribution in the body after injection into the tail was comparable to delayed results of intravenous injection; this resemblance was interpreted by high vascularization of the tail. (5) It was concluded that the method of serial tail amputation may be useful for outlining the dynamics of isotopes spread in bodies of experimental animals.