Abstract
Summary
A method and an apparatus for routine electroejaculation of guinea pigs were developed, the semen coagulum or “vaginal plug ”examined, and the coagulation process described. A series of proteolytic enzymes were tried for their liquefying activity and 0.1% chymotrypsin was found to result in consistent liquefaction and good sperm motility. Mean ejaculate volume was 0.5 ml and mean motility at 39°C was 51%. There was no evidence of gross morphological or histological changes in the reproductive system of guinea pigs electroejaculated at biweekly intervals.
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