In vitro Incorporation and Hydroxylation of Lysine-2-C 14 In Rat Biopsy-Connective Tissue. ∗

Abstract

Summary

1. Lysine-2-C¹⁴ becomes incorporated and hydroxylated in connective tissue incubated in Krebs-Henseleit medium for 12 hours. 2. Specific activity of collagenous and non-collagenous protein in the tissue studied in vitro was greater than that in tissue studied in vivo. 3. Hydroxylation of labeled lysine appears to occur more effectively in connective tissue of rats given labeled lysine intraperitoneally than in the connective tissue incubated with lysine-2-C^14.