Abstract
Summary
1. Infectious canine hepatitis (ICH) virus grown and passed 39 times in tissue cultures derived from wild raccoons. The tissue culture virus in 39th passage was neutralized by specific immune serum prepared against the original dog spleen virus. When the 39th passage virus was introduced into antibody free puppies, all developed a mild ICH infection resulting in appearance of specific serum neutralizing antibodies in blood against dog kidney tissue culture virus. 2. Eight percent of the puppies, 3-7 months of age, obtained from farms in Central and Northwest Kansas, have specific neutralizing antibodies against ICH virus; ICH antibodies in a 1:5 titre were found in 1 of 23 sera from wild raccoons. 3. ICH virus was isolated from dog kidney tissue cultures derived from 2 apparently healthy dogs. In one instance, specific ICH neutralizing antibody was present in the blood.
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