Abstract
Summary
A chemical determination of INH in patient's blood has been devised which can be carried out within one hour. The serum is deproteinized with 4 volumes of alcohol, centrifuged, and quantities corresponding to one cc serum are heated with 0.5 cc 0.4% 1-fluoro-2.4-dinitrobenzene solution in alcohol and with 0.1 g borax for 5 minutes at 80°. The developed color is read at a wave length of 500 mμ. The method gives good agreement with results obtained by the microbiological methods within the errors allowed for clinical purposes.
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