Abstract
Summary
When Walker carcinosarcoma 256 cells were grown under clonal growth technics. 50-100% plating efficiency was noted in a medium containing whole bovine serum. When dialyzed serum was used in the medium, few or no colonies were formed. Addition of pyruvate, α-ketoglutarate, and/or oxalacetate to the substrate resulted in plating efficiencies of 90-100%. Activity of these 3 metabolites was the same irrespective of addition of individual compounds or combinations thereof. Other Kreb's acids and metabolites tested failed to promote survival and growth of isolated Walker cells.
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