Abstract
Summary
The opacimetric and hematocrit methods for measuring relative mitochondrial volume are compared. Data from mitochondria swollen by exposure to hypotonic sucrose solutions demonstrate the validity of the simple opacimetric method, since, as osmolarity decreases, rate of increase in mitochondrial pellet volume approximates rate of decrease in optical density of the mitochondrial suspension. The results are discussed in terms of a current dispute regarding permeability of the mitochondrial membrane to sucrose.
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