Indirect Staining Reaction with Fluorescent Antibody for Detection of Antibodies to Pathogenic Fungi

Abstract

Summary

Antibodies in sera from patients with systemic mycoses were demonstrated by the indirect method of staining with fluorescent antibody. In spite of a general auto-fluorescence of pathogenic fungi, addition of human antiserum and a fluorescein-labeled rabbit antihuman gamma globulin resulted in characteristic fluorescing reactions. In the case of cryptococcosis antibodies were demonstrated by the fluorescent technic but not by other serologic methods.