Abstract
Summary and conclusion
Properties of the fluorescent dye DNS for labelling proteins have been described. Investigations of conditions of DNS binding to serum proteins revealed ratios of 3-4 M DNS/M gamma-globulin and about 7 M DNS/M albumin. Dealing with the use of DNS in immunochemistry, experiments have been carried out with the ADH-rabbit antiserum system, measuring enzyme inhibition by the Ab. Labelling of Ab with DNS did not affect rate of formation of Ag-Ab complex and had no influence on quantity of formed precipitate. The reported data suggest that because DNS does not affect the Ag-Ab reaction, it can be successfully applied in histochemical studies.
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