Enzyme and Nucleic Acid Content of Thrombocytes from Normal and Thrombocytopenic Calves. ∗

Abstract

Summary

1. Bovine thrombocyte concentrates contained acid phosphatase, beta-D-glucuronidase, arylsulfatase, aliesterase (active with three fatty acid esters of carbon chain lengths: C₂, C₁₂ and C₁₈) and acetyl-cholinesterase but no alkaline phosphatase. Benzoylcholine was not hydrolyzed by thrombocytes. 2. There was no significant difference in these enzyme activities of thrombocytes from normal or thrombocytopenic calves calculated in terms of activity per 10¹⁰ thrombocytes. 3. There was no significant quantity of deoxypentosenucleic or pentosenucleic acid in bovine thrombocyte concentrates from either normal or thrombocytopenic calves. 4. Thrombocytes released by hypoplastic bonemarrow could not be differentiated from normal thrombocytes by the tests used.