Abstract
Summary
Urinary metabolites of 2-methyl-1,4-naphthoquinone have been separated by paper chromatography and their response to various hydrolytic procedures studied. Three conjugates, phosphate, glucuronide and sulfate were hydrolyzed both by hydrochloric acid and by ceric sulfate. The sulfate was hydrolyzed to a greater degree by hydrochloric acid and this reagent was less effective than ceric sulfate in splitting phosphate and glucuronide. Following treatment of rat urine with hydrochloric acid or with ceric sulfate, significant quantities of sulfate remained unhydrolyzed. A combination of these procedures hydrolyzed the sulfate. 2-methyl-1, 4-naphthoquinone was released from glucuronide and sulfate after incubation in the presence of Ketodase. The conjugate present in lowest relative concentration was phosphate, that present in largest amount was glucuronide.
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