Inhibition of Glucose Uptake by Acidosis in vitro. ∗

Abstract

Summary

Glucose uptakes of rat diaphragms in vitro were compared at pH 7.4 and 6.8. The acid medium inhibited glucose uptake but the degree of inhibition could be decreased by adding insulin to both media. At insulin concentrations in excess of 0.08 i.u./ml, glucose uptake was the same at each pH. With 0.008 i.u./ml insulin a pH of 7.1 was almost as inhibitory as pH 6.8. There was no enhancement of glucose uptake at pH 7.8.