Abstract
Summary
1) A new fluorescent label, tetramethylrhodamine isocyanate, is described and its use demonstrated in the immunohistochemical fluorescence technic. The label fluoresces orange and is of particular value for staining tissues which show a green auto-fluorescence similar to fluorescein fluorescence even when unstained. 2) Serum from a patient with chronic thyroiditis combined with normal thyroid tissue in the colloid region and perhaps in the glandular epithelium, while normal human serum showed no such staining. Thyroid from a patient with chronic thyroiditis, infiltrated with lymphocytic cells, was stained by rabbit antibody to human globulin in the region occupied by the infiltrating cells. Normal lymph node showed no such staining. This indicates that the infiltrating cells contained human globulin and may have been important in the destruction of the thyroid during the course of the disease.
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