Distribution of Sulfur in Regenerating Wound Tissue

Abstract

Summary

1. Following pre-labeling of labile body proteins by injection of S-³⁵-L-methionine and subsequent wounding, regenerating tissue was removed at 4 and 6 day intervals, fractionated, and assayed chemically and radioactively for bound and nonprotein bound cystine and methionine, and for free and mucopolysaccharide sulfate. 2. Specific activity of unfractionated wound cystine and methionine was studied at intervals over 3 weeks. 3. The data indicate that, for the first 6 days post-injury, S³⁵ administered as S³⁵-L-methionine 2 days prior to wounding is incorporated into wound tissue in large measure as protein-bound methionine and cystine. 4. During the interval studied, administered methionine-S³⁵ is not a precursor for wound tissue mucopolysaccharide sulfate.