Metabolism of Human Amnion Cell Cultures Infected with Poliomyelitis Virus. I. Glucose Metabolism During Virus Synthesis. ¶

Abstract

Summary

1. A typical growth curve of poliovirus type II in human amnion cell cultures is reported. 2. During the early hours of virus multiplication and prior to onset of cytopathogenic effect human amnion cells showed a higher uptake of glucose than uninfected controls. This uptake was retarded with the active release of virus into the culture fluid. 3. Phosphorus was taken up at an increased rate by cells soon after infection, and was released back into the medium with the rise in virus titer. 4. Inhibitors of the oxidative pathways of glucose metabolism affected neither cell maintenance nor virus reproduction. It is assumed that glycolytic rather than oxidative processes are important for reproduction of poliovirus within human amnion and monkey kidney cell cultures.