Biochemical Changes in HeLa Cells Associated with Infection by Type 2 Adenovirus

Abstract

Summary

Infection of HeLa cells with a large inoculum of adenovirus type 2 produced a rapid and prolonged stimulation of cellular uptake of the radioactivity of inorganic P³² and glycine-2-C¹⁴, with no change in concentration of P, RNA, or DNA. In fractions taken 5 hours after inoculation, the increased rate of P³² uptake was observed in each of the biochemical fractions tested, minimally in lipid. Glycine uptake was increased only in the acid-soluble fraction. In contrast to the early alteration of radioisotope uptake, increased production of lactic acid was not observed until 8 to 24 hours after infection.