Abstract
Summary
1. Feeding of a high fat diet increased the alkaline phosphatase activity of rat liver during the absorptive period. This activity returned to normal after a 12-hour fast. Adaptation to a high fat diet for 30 days did not alter this pattern. 2. These results indicate that the increase of alkaline phosphatase activity after feeding of fat is a transitory result of fat absorption and not a consequence of change of metabolic pattern due to fat adaptation. 3. The method using p-nitrophenol phosphate as a substrate was modified for determining phosphatase activity in tissue homogenates.
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