Abstract
Summary
1. The induction time of pentobarbital narcosis was shortened by 50% when carried out in the presence of calcium binding substances. 2. Removal of calcium by binding with citrate ion or pretreatment of the fish in calcium-free Ringer's solution prior to induction of pentobarbital narcosis resulted in a prolongation of the induction time (90%). 3. Balanced calcium EDTA solution shortened the induction time (30%) and lengthened the recovery time (100%) in spite of its theoretical inability to chelate calcium, while excess EDTA caused a greater shortening in induction time (50%) and lengthening in the recovery time (200%). 4. Excess calcium exerted no effect on induction or recovery of pentobarbital narcosis.
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