Abstract
Summary
1. The effect of acetoacetate injections in doses of 500 mg/kg on ascorbic and dehydroascorbic acid contents of blood and tissues, and their excretion in urine, was studied. 2. Animals injected with aceto-acetate excrete more dehydroascorbic acid and less ascorbic acid. Their tissues (liver, kidney, spleen and adrenal) also accumulate more dehydroascorbic acid, and the amount of ascorbic acid in them is diminished. 3. Glucose-cyclo-acetoacetate or its hydrolysed product has been found to prevent this increased accumulation of dehydroascorbic acid in blood and tissues. 4. Acetoacetate has been shown to cause oxidation of ascorbic acid to dehydroascorbic acid in vitro in presence of a few drops of CuSO4 solution at a faster rate than that in the control. 5. The mechanism for prevention of acetoacetate-induced accumulation of dehydroascorbic acid by hydrolysed glucose-cyclo-acetoacetate has been suggested.
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