Abstract
Summary
A procedure is presented for determination of Tolbutamide in plasma. The method consists of a chloroform extraction of weakly acidified plasma, concentration of the extract to dryness, dissolution of the residue in a measured volume of ethanol, treatment of the resulting solution with Darco G-60, and measurement of the absorbance of the resulting charcoal filtrate at 228 mμ. Recoveries of Tolbutamide from human and dog plasmas have been satisfactory and plasma levels determined by this procedure have correlated well with the hypoglycemic response elicited by the drug.
Get full access to this article
View all access options for this article.