Abstract
Summary
1) Our data indicate that antibody against complement can be expressed as protein in specific combination with an immune precipitate to which complement has been affixed. 2) Anti-complement which has been labeled by injection of glycine-1-C14 into animals during active antibody production can be demonstrated as carbon-14 bound to precipitate-complement complexes. 3) Electrophoretic analysis of the sera of rabbits before and after immunization with guinea pig, human, and mouse complement shows that a consistent rise in γ globulin occurs during the immunization process. 4) Finally, electrochromatographic separation of antibody containing sera proved to be an effective means of concentration and partial purification of the antibody.
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