Group A Hemolytic Streptococci. I. A Chemically Defined Medium for Growth from Small Inocula

Abstract

Summary

1) Defined media which allow heavy growth of 3 strains of group A streptococci have been developed. The medium consists of either a) 21 amino acids, glutamine, 6 vitamins, salts, purines, pyrimidines and glucose or b) 13 amino acids and 4 vitamins. 2) Cysteine is important both as an essential amino acid and as a reducing agent. As an amino acid only a small amount (10 μg ml) is needed and this can be substituted by an equivalent amount of cystine. As a reducing agent it can be replaced by ascorbic acid and less effectively by thiomalic or thioglycollic acids. Concentration of cysteine was critical for initiation of growth from small inocula. With less than 5 × 10⁶ ml cells at least 350 μg ml cysteine HCl are needed for obtaining visible growth. 3) Pyridoxal was necessary in a medium of 13 amino acids and 3 vitamins (nicotinic acid, pantothenate, riboflavin) whereas in a complete medium (22 amino acids) no need for pyridoxal was found. Pyridoxal could be replaced by L- or DL-alanine.