Preparation of Partially Purified Porcine Intrinsic Factor

Abstract

Summary and Conclusions

A simple method is described for preparation of highly purified intrinsic factor PI from porcine stomach pyloric mucosa. In pernicious anemia patients in relapse, 35 mg of intrinsic factor PI when given with 15 μg vit. B₁₂ is defined as one U.S.P. oral unit. Dialysis of intrinsic factor PI gives a material with a potency of about 15 mg/U.S.P. unit. In the urinary excretion test, one-half U.S.P. unit of intrinsic factor PI gives significant and measurable urinary excretion of vit. B₁₂ Co⁶⁰ when given orally with 1 μg vit. B₁₂ Co⁶⁰. Approximately 1.5 U.S.P. units of intrinsic factor PI will give maximum urinary excretion of vit. B₁₂ Co⁶⁰. Intrinsic factor PI can be further purified on a DEAE-cellulose ion exchanger to give a material with an estimated potency of 9 mg/U.S.P. unit.