Abstract
Summary
Following an essentially alternating passage method between HeLa and bovine embryo kidney cultures, infectious bovine rhinotracheitis (IBR) virus was adapted to HeLa cells and propagated through 26 serial transfers. The adapted virus rapidly destroyed HeLa cells and yielded, when measured in bovine cultures, virus titers comparable to those of bovine kidney-grown virus. It was specifically neutralized by an immune guinea-pig serum prepared against bovine-grown virus and, although of somewhat lesser virulence to calves than the parent virus, it induced a comparable level of neutralizing antibodies in all animals and afforded them a solid protection against a virulent challenge.
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