Abstract
Summary
Normal tissues from trout—a coldwater teleost—have been successfully cultured for as long as 65 days at 19°C or lower in 20% serum, 30% SM 199, 45% Earle's solution and 5% embryo extract. Excellent results were obtained with human cord, human, homologous sera or bovine amniotic fluid when used at 20% level by volume. Manipulation and incubation temperatures below 22°C are thought to be essential.
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