Abstract
Summary
(1) A method has been described whereby pantetheine and free pantothenic acid may be determined microbiologically in a medium in which the 2 factors exert (on a molecular basis) the same effect on Lactobacillus arabinosus. The action of the 2 growth factors is additive. (2) The CoA bound pantothenic acid is liberated as pantetheine by incubation of an aqueous tissue extract with intestinal phosphatase and can be determined in the presence of preformed free pantothenate. (3) The results of this method, applied to several tissue extracts, agree with those of Novelli and Lipmann.
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