Accumulation of Organic Acids by HeLa Cells Infected with Type 4 Adenovirus. ∗

Abstract

Summary

Fluids from cultures infected with type 4 adenovirus were markedly more acid than fluids from non-infected control cultures. The lowered pH of infected cultures resulted from an accumulation of lactic, pyruvic, acetic and alpha ketoglutaric acids. The acids were separated by chromatography on a celite column, and measured by colorimetric and acid-base titration technics. Confirmation of the identity of the organic acids was obtained by paper chromatography. Concomitant with the increased accumulation of acid in fluids of infected cultures, an increased utilization of glucose by cells of the virus-infected cultures occurred.