Transfer of Lymph Node Cells to Recipient Rabbits Pre-Injected with Blood Leucocytes of Donors. †

Abstract

Summary

As observed in previous studies the transfer to irradiated recipient rabbits of donor lymph node cells incubated in vitro with soluble antigenic material derived from Shigella paradysenteriae was followed by the appearance of agglutinins to these bacilli in the sera of the recipients. If, 4–6 days prior to cell transfer, the recipients were injected intradermally with leucocytes obtained from blood or from peritoneal exudates of the donors, agglutinins failed to appear in the recipients' sera, or appeared in low titer. This effect could be brought about consistently with the pre-injection of 10⁶ to 100 × 10⁶ leucocytes; not by the pre-injection of rabbit erythrocytes or of leucocytes of horse, cow and chicken blood; and occasionally by the pre-injection of human blood leucocytes.