Abstract
Summary
A microbiological method for biochemical screening of compounds of potential interest in the chemotherapy of leukemia, using mutants of B. subtilis resistant to amethopterin and E. coli mutants resistant to purine-antagonists, has been described. Greater growth suppression by a test compound in the resistant strain, when contrasted to the parent strain, has been sought (collateral sensitivity). This phenomenon of collateral sensitivity has been observed for several chemical analogs of the pyrimidine moiety of thiamine. Possible significance of these findings for studies in intermediary metabolism and for cancer chemotherapy has been discussed.
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