Abstract
Summary
(1) These studies further evaluate whole blood as sources of essential amino acids for protein depleted adult male rats. Human and canine blood again were rejected and failed to support repletion. Human blood supported rapid repletion when supplemented with 55 mg L-isoleucine and 15 mg DL-methionine per 120 mg of blood-N. Less than half these levels added to plasma supported repletion. Plasma alone permitted an intermediate rate of repletion. (2) Feeding normal dogs a non-protein diet and 150-375 mg N /kg/day as lyophilized human or dog blood did not increase the blood urea-N or NPN or produce negative N balance. Force-feeding 12-25 ml of citrated human blood increased blood urea-N of fasting rats. Supplementing the blood with isoleucine and methionine, however, did not reduce the blood urea-N. (3) Analysis of human blood, human plasma, and dog blood for 13 amino acids is reported.
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