Abstract
Summary
1. Two modified blood mediums, one diphasic, the other liquid, are described for growth and maintenance of Entamoeba histolytica of large and small races. The mediums are modifications of the one described and used by Tarshis(11) to grow Mycobacterium tuberculosis. 2. Strains of small race-Griffith; Brown, a new strain; Conrad; and 110-maintained in the diphasic medium exhibited increased motility as compared to egg slant controls and increased growth over liquid charcoal cultures. The large race strains-200; K9; and Komin, a new strain-grew equally well in the diphasic blood medium. 3. The liquid blood medium was particularly unsatisfactory for maintenance of strains of small race, as the organisms were not easily discerned in the highly cellular sediment. 4. No attempts were made to culture stools or to isolate new strains of small or large race in either blood medium. 5. A 48-hour subculturing scheme was satisfactory for diphasic blood medium, liquid charcoal medium and egg slants; 0.5 ml of sediment was transferred.
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