Abstract
Conclusions
A method for determination of pregnanetriol is presented. The method seems to be reproducible with adequate recoveries and specificity. Recoveries average 78.5%. Specificity is assured by chromatographic purification, oxidation, and determination of pregnanetriol as ketogenic steroid. The spectrum of material from the florisil colurrm in sulfuric acid was authentic with the one obtained from pure pregnanetriol (Fig. 1). In the oxidized sample the Zimmermann color obtained was purple with distinct peak at 520 mμ (Fig. 2). The unoxidized sample showed brown color with the Zimmermann reagent and gave a linear absorption curve.
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