Abstract
Summary
A colorimetric assay method for measuring neutralizing antibodies against adenovirus in HeLa cell tissue culture in plastic panel cups is described. Advantage is taken of the fact that adenovirus growth in HeLa cell cultures lowers the pH of the medium below that of uninfected cultures. The reaction is standardized so that in 3 days the phenol red indicator has turned yellow by unneutralized virus while the indicator has turned only to red orange by the metabolism of the HeLa cells when the virus antigen is neutralized. It has been shown that this colorimetric test gives titers in paired serums from human adenovirus infections with antigen types 1 through 10 which are similar or slightly higher than those obtained with a test tube method depending for reading on microscopic observation of cytopathogenesis. This method has also been found suitable for typing adenoviruses.
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