Abstract
Summary
Two methods for the assay of small amounts of insulin are presented. Both involve using the alloxan diabetic hypophysectomized mouse. One involves measuring the fall in blood sugar in test animals over a 20 minute period after the intravenous administration of insulin, the insulin being given one hour after the subcutaneous injection of 25 mg glucose. The other method involves measuring the fall in blood sugar 30 minutes after the intraperitoneal administration of the insulin, which, as in the intravenous method, is given one hour after a 25 mg glucose meal. The intravenous method, which is the more sensitive of the two, is capable of detecting as little as 250 μU of insulin.
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