Abstract
Summary
A new colorimetric procedure is substituted for microtitration in the measurement of ammonia by the Conway method. The ammonia is oxidized by addition of hypobromite, and the excess hypobromite determined by its power to decolorize phenosafranin. The range of the method as described is 0.05 to 0.5 micromole. The procedure has been applied to brain tissue and to blood, and to the measurement of glutamine. It has also been used for the determination of non-protein nitrogen after Koch-McMeekin digestion, the microdiffusion being omitted.
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